PCR Reagents and Kits

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit

Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.

Thermo Scientific™ T4 DNA Polymerase (5 U/μL)

Thermo Scientific™ T4 DNA Polymerase (5 U/μL)

Ensure accuracy in DNA synthesis with template-dependent T4 DNA Polymerase that catalyzes the 5'-3' synthesis from primed single-stranded DNA.

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific™ Phusion™ High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Invitrogen™ Yeast tRNA (10 mg/mL)

Invitrogen™ Yeast tRNA (10 mg/mL)

Invitrogen Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10 to 20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.

TaqMan™ TaqMan™ Gene Expression Master Mix

TaqMan™ TaqMan™ Gene Expression Master Mix

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Invitrogen™ DEPC-Treated Water

Invitrogen™ DEPC-Treated Water

Ambion DEPC-treated water is certified nuclease-free. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC.

Thermo Scientific™ Phusion Green HF Buffer Pack

Thermo Scientific™ Phusion Green HF Buffer Pack

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel.

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific™ Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.

TaqMan® TaqMan™ Gene Expression Assay (FAM)

TaqMan® TaqMan™ Gene Expression Assay (FAM)

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)

Invitrogen™ Gel Loading Buffer II (Denaturing PAGE)

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue.

Thermo Scientific™ Phire Green Hot Start II DNA Polymerase

Thermo Scientific™ Phire Green Hot Start II DNA Polymerase

A novel hot start DNA Polymerase for routine and high throughput PCR applications requiring speed and specifity.

Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)

Invitrogen™ GlycoBlue™ Coprecipitant (15 mg/mL)

GlycoBlue Coprecipitant consists of a blue dye covalently linked to glycogen, a branched chain carbohydrate, which is useful as a nucleic acid coprecipitant.

Invitrogen™ Platinum™ <i>Taq</i> DNA Polymerase

Invitrogen™ Platinum™ Taq DNA Polymerase

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

Invitrogen™ RT-PCR Grade Water

Invitrogen™ RT-PCR Grade Water

RT-PCR Grade Water is certified free of nucleases, and free of nucleic acid contamination that may cause false-positive signals in RT-PCR. The RT-PCR Grade Water is ready to use and requires no preparation, mixing, or autoclaving.

Invitrogen™ SSC (20X), RNase-free

Invitrogen™ SSC (20X), RNase-free

Molecular biology grade, Invitrogen 20X SSC solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use.

Invitrogen™ BSA, acetylated (20 mg/mL)

Invitrogen™ BSA, acetylated (20 mg/mL)

Invitrogen Acetylated BSA is used as a carrier protein and as an enzyme stabilizer in which the absence of contaminants such as nucleases and proteases is essential.

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Invitrogen™ Nuclease-Free Water (not DEPC-Treated)

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving.

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR

TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms. It provides best in class performance including the widest quantifiable range, in both single and duplex reactions, even with challenging targets.

Invitrogen™ AccuPrime™ <em>Taq</em> DNA Polymerase, high fidelity

Invitrogen™ AccuPrime™ Taq DNA Polymerase, high fidelity

Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

Thermo Scientific™ Phusion Green High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific™ Phusion Green High-Fidelity DNA Polymerase (2 U/μL)

Thermo Scientific Phusion High-Fidelity DNA Polymerases set a gold standard for high performance PCR.

Maxima Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Maxima Maxima SYBR Green qPCR Master Mix (2X), with separate ROX vial

Ready-to-use solution optimized for qPCR and 2-step RT-qPCR.

Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free

Invitrogen™ PBS - Phosphate-Buffered Saline (10X) pH 7.4, RNase-free

PBS (phosphate buffered saline) is a pH-adjusted blend of ultrapure-grade phosphate buffers and saline solutions.

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

Applied Biosystems™ TaqMan™ Universal PCR Master Mix

TaqMan Universal PCR Master Mix is the ideal reagent solution when you need a master mix for multiple 5' nuclease DNA applications.

Invitrogen™ TE, pH 8.0, RNase-free

Invitrogen™ TE, pH 8.0, RNase-free

Invitrogen molecular biology grade, TE, pH 8.0 solution is supplied in one bottle containing 500 mL. The buffer is certified RNase-free, economical, and ready-to-use.

Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

Applied Biosystems™ TaqMan™ Universal Master Mix II, no UNG

TaqMan Universal Master Mix II is a convenient mix of components (except primers, probes, template, and water) necessary to perform a real-time polymerase chain reaction (PCR).

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, with separate ROX vial

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Applied Biosystems™ Human PGK1 (Phosphoglycerate Kinase 1) Endogenous Control (VIC™/MGB probe, primer limited)

Applied Biosystems™ Human PGK1 (Phosphoglycerate Kinase 1) Endogenous Control (VIC™/MGB probe, primer limited)

Human PGK1 (Phosphoglycerate Kinase 1) Endogenous Control (VIC™/MGB probe, primer limited)

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer I

Chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation

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